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We also showed that these cell lines had AP activity and expressed pluripotent markers such as Oct4, Sox2, and Nanog, The cell lines also formed EBs, revealed normal karyotypes, and were capable of differentiating into the three germ layers.
The transplanted sphere-derived cells did indeed give rise to cells from all three germ layers.
The stem cell lines generated in the present study appear to have the same characteristics as other hESC lines, including expression of the same markers of pluripotency, self-renewing capacity, genetic stability, and ability to differentiate into derivatives of all three germ layers of the body.
We have demonstrated a method of creating parthenogenetic human embryonic cells" and that such cells "can be differentiated in vivo into the three germ layers that lead to all cell types found in a human body," reported Dr.
2] Some of these trapped cells are totipotential blastomeres that can develop into any of the three germ layers.
These proprietary human PluriCell lines display many of the usual characteristics of human ES cells, including the ability to differentiate into cell types of the three germ layers.
The ES cells differentiated into derivatives of all three germ layers both in vitro and in chimeric offspring and teratomas.
5, 1998--Human pluripotent stem cells -- cells with the demonstrated ability to differentiate in vitro into all three embryonic germ layers -- have been successfully derived in culture by scientists at the Johns Hopkins University School of Medicine, according to a paper to be published in the November 10 issue of Proceedings of the National Academy of Sciences.
During gastrulation, the embryo folds inwards into a cup-like shape, forming the primary germ layers that give rise to all the organs in the body.