Rat fetal (e15.5; n = 13-30, from 20 litters) testes and ovaries were cut into ~ 1-[mm.sup.3] pieces and cultured as described above for human hanging drop
(d) Hanging drop
culture of ntMSCs stimulated Shh expression.
Gliding motility was checked under a microscope (magnification, x 100) by using a hanging drop
preparation of a 48 hours liquid medium culture.
I'm sure improvements in techniques have been made since I did this in the late 1960s, but basically once the mammalian culture medium with an antibiotic was diluted to amphibian tonicity with sterile distilled water, a small piece of lung from an adult or late embryo frog or salamander was placed on a culture coverslip (explant method of obtaining cells--no enzymes needed for cell separation), which was then configured in a hanging drop
technique with a larger coverslip covering the relatively large depression in a Romicon or Maximow slide.
Outside-half Shaun Connor booted a high and hanging drop
goal between the posts before Glasgow had full-back Bernardo Stortoni sin-binned when he deliberately knocked-on as the Ospreys mounted a quick attack past the Scottish region's 22.
phaseolina was introduced into soybean stems by puncturing a hanging drop
from a prepared sclerotial suspension or by adding a cornmeal/sand culture mixture into potting soil prior to planting soybean in a greenhouse.
Koch invented the "hanging drop
" preparation using ox-eye aqueous humor to grow the bacilli.
HDI also markets Diascan-S, a wipe system that is ideal for those who cannot attain a hanging drop
of blood or who have shaky hands, and Diascan Partner, Diascan-S with an attached voice synthesizer for the visually impaired.
Hoop earrings - along with hanging drop
types - have been banned by the rector of Glen Urquhart High School, in Drumnadrochit, on the shores of Loch Ness.