X = enzymatic extract volume (mL) or mass of immobilized
(g) used in the reaction; t = reaction time (min).
In this study, urease was isolated from Aspergillus fumigatus and immobilized
in calcium alginate beads for the first time in literature.
Figure 3 shows the effect of performing the immobilization process under different pH on the catalytic and the retained activity of immobilized
Afterward, the immobilized
cellulases were washed thoroughly with 0.
Immobilization efficiency was defined as the effectivity of enzyme immobilized
on the support used from the total enzyme introduced, which was calculated as formula referred .
Thermal stability of soluble and immobilized
enzymes: The thermal stability was determined by preserving at various temperatures (range 10-60[degrees]C) sealed flasks with soluble and immobilized
enzymes for 2 h in the standard buffer (50 mM [Na.
Different concentrations (2, 3 and 5% w/v) of sodium alginate immobilized
cell beads were used for production of glutamic acid using the above mentioned optimized medium.
Additionally, immobilization of [beta]-galactosidase on CA-PMMA might have reduced its ability to undergo conformational changes that are intrinsic to enzyme-substrate interaction, thereby decreasing maximum reaction rate for the immobilized
The effects of optimum pH on the free and immobilized
LX-1 [alpha]-galactosidase were investigated by assaying both preparations in the pH range of 3 to 8.
0 min respectively which were lower than those of immobilized
The objectives of this study were to provide a description of blood chemistry parameters and serum cortisol levels in hunted, tame, and chemically immobilized
muskoxen and to examine differences in blood chemistry parameters and levels of stress associated with different capture techniques.
Activity of antibiotic increased for immobilized
cells just after 4 hours of incubation, which shows that immobilization was a better process, as compared to free cell production of antibiotics.