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Counterimmunoelectrophoresis and immunodiffusion for the detection of antibodies to soluble cellular antigens.
Serologic testing may assist in diagnosis, but complement fixation and immunodiffusion lack sensitivity and the WI-1 antigen-based antibody test has good sensitivity and specificity but is not widely available.
They used a radial immunodiffusion method with polyclonal antiserum to measure urinary L-PGDS ([beta]-trace); for that assay, all urine samples had to be concentrated more than 100-fold.
Farm sampled positive Ct A 26 3 31.4 43 1 34.8 19 0 42 0 B 30 3 34.9 47 6 27.1 * AGID, agar gel immunodiffusion assay; rRT-PCR, real-time reverse transcription-PCR to detect matrix protein; Ct, cycle threshold values of positive results (averages).
[3] Nonstandard abbreviations: BJ, Bence Jones; FLC, free light chain; PE, protein electrophoresis; IFE, immunofixation electrophoresis; RID, radial immunodiffusion assay; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; SLE, systemic lupus erythematosus; and RF, rheumatoid factor.
Acute histoplasmosis usually can be diagnosed by serologic tests (immunodiffusion and complement fixation) and sometimes by positive sputum culture or lung biopsy culture.
Occasionally, [approximately equal to] 10% of the time, serum samples were tested by a single commercial laboratory, which used immunodiffusion testing for precipitin-type or complement fixing-type antibodies (6).
Other assays for quantifying the serum concentration of Gc include radioimmunoassay (19), radial immunodiffusion (20), rocket immunoelectrophoresis (21), and ELISA (22).
Acute coccidioidomycosis can be diagnosed by serologic tests for immunoglobulin M (IgM) detection (such as tube precipitin, enzyme immunoassay, latex agglutination, or immunodiffusion), and immunoglobulin G (IgG) detection (such as immunodiffusion or complement fixation) in the presence of pneumonia or erythema nodosum and occasionally by positive sputum culture (4).
patients EIA IgM EIA IgG ID CF CF titer Negative serologic results: completed 2 tests 11 - - - - 5 ND ND - - 3 - - - - 6 - - - - 1 ND ND - - 1 - - ND ND 1 ND ND - - 1 - - ND ND Negative serologic results: completed 1 tests 10 ND ND ND ND 4 ND ND ND ND 6 ND ND ND ND Positive serologic results: completed 1 tests 2 + - + - 1 + - + + 4 1 - + - - 1 - - + - 1 - + + + 16 Positive serologic results: completed 1 tests 3 ND ND ND ND 1 - - - + 2 * EIA, enzyme immunoassay; Ig, immunoglobulin; ID, immunodiffusion; CF, complement fixation; ND, not done; -, negative test result; +, positive test result.
Radial immunodiffusion (RID) is simple to perform but time-consuming.
Agar gel immunodiffusion is a serologic assay used to detect antibodies to influenza viruses.