The procedure of Thouas et al11 was carried out for the differential staining of inner cell mass
and trophectoderm cells.
Cell allocation to the inner cell mass
and the trophectoderm in bovine embryos cultured in two different media.
The larva of this stage consists of a peripheral layer of pinacocytes and an inner cell mass
They would then take out the stem cells from its inner cell mass
and dope them with the appropriate hormones and proteins to turn the stem cells into, say, heart tissue, which could then be used to repair your ailing heart.
In the latter case, the line between cellular life and organismic death is less clear-cut; in removing the inner cell mass
from a blastocyst, the Thomson method is seen as killing an embryo that is still alive, albeit certain to die because discarded.
It does this by secreting protein-digesting enzymes that digest the outermost portion of the uterine wall, enabling it to bury itself there, with the side containing the inner cell mass
, the "blastoderm," coming into contact first.
This permits the establishment of apico-basal polarity and thereby the 1st fate specification of the blastomeres: trophectoderm derives from blastomeres with a large apical membrane at the cell-medium interface; whereas blastomeres composing the inner cell mass
descend from cells with comparably larger basal cell-cell interfaces.
The total cell number, as well as differential cell numbers representing the inner cell mass
(ICM) and trophectoderm were determined by a differential staining technique for blastocysts.
This low success rate is assumed to be a cumulative result of losses at different stages of pregnancy due to several unknown causes including chromosomal abnormalities, low cell number and altered inner cell mass
(ICM) rate of blastocysts, irregular-sized blastomeres, and cytoplasmic fragmentation (Wang et al.
Embryonic stem cells are derived from the inner cell mass
of blastocysts, which are hollow balls of cells that form in early development.
Worcester, MA) announced the decision of the Board of Patent Appeals and Interference, interference number 105,192, between itself and Geron Corporation over methods of producing cultured inner cell mass
(CICM) cells, often referred to as embryonic stem cells.
Hwang's team made history when they removed the inner cell mass
(cells that become the embryo) from the blastocyst and grew the cells in a nutrient-rich petri dish.