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1] Nonstandard abbreviations: DGGE, denaturing gradient gel electrophoresis; TTGE, temporal temperature gradient gel electrophoresis; IVS, intervening sequence (intron); RDB, reverse dot blot; and nt, nucleotide.
The probe was designed to anneal with the 3 loci and have 2 intervening sequences of 22 and 55 nt (1).