Peptide Average log P Average log S PGKYKLAVSEK -2.69 -4.82 PGKFYVSSGATTFPAVYL -0.40 -6.28 QVGFSSTAMWNKTFDVSGATQ -2.47 -4.75 QTAMRMIFTALTSPS -2.50 -4.49 SGVQPDAPSNDSKDT -12.45 (+-6.32) -1.82 PSTLSVSSGTVYEKHEGLS -1.92 -3.95 KREAKQEE -6.03 (+-2.50) -3.35 PGPTVFPAVLY 1.70 (+-1.45) -5.16 Peptide Water solubility Isoelectric point
PGKYKLAVSEK Good pH 10.05 PGKFYVSSGATTFPAVYL Poor pH 9.52 QVGFSSTAMWNKTFDVSGATQ Poor pH 6.75 QTAMRMIFTALTSPS Poor pH 11.04 SGVQPDAPSNDSKDT Good pH 3.59 PSTLSVSSGTVYEKHEGLS Good pH 5.3 KREAKQEE Good pH 7.15 PGPTVFPAVLY Poor pH 5.93 Properties such as log P (octanol-water coefficient), log S (solubility), and isoelectric point
determine the oral absorption capability of oligopeptides.
The isoelectric points
(pI) as calculated for the AA were 3.89 (aril) and 4.03 (seed) (Table 2).
Table 1 Isoelectric points
(pI) of the hemoglobin variants.
IEF allows the separation of Hb variants with isoelectric points
that differ by as little as 0.02 pH units.
of ESBL enzymes from a few isolates (n = 6) were noted for typing of ESBL by isoelectric focusing.
Soil properties such as active/free iron oxide ratio, isoelectric point
(IEP), cation exchange capacity (CEC), and potassium-calcium cation exchange equilibrium have been related to OM content in Chilean volcanic soils.
Because histidine has an isoelectric point
of 7.6 and histamine has an isoelectric point
of 8.4, a buffer of pH 8 was used for maximum separation.
(7,10,24) Two-dimensional electrophoresis is a method used to separate proteins by their isoelectric point
in the first dimension, and subsequently by molecular weight in the second dimension.
The influence of these pretreatments to cotton fabric interface phenomena was investigated throughout the change of fabric surface free energy, zeta potential, Isoelectric Point
, Point of Zero Charge, and specific amount of surface charge.
The particles comprise a silica-based core and an at least a discontinuous layer of an oxide, hydroxide or hydrated oxide of zirconium, whose isoelectric point
is greater than that of the core.
Available July I and co-developed with DiagnoSwiss SA, the OFFGEL Fractionator fractionates intact proteins and peptides by isoelectric point
and can run samples in parallel.
Separation can be done simply at the whole-protein level using two-dimensional gel electrophoresis; this technique separates molecules by molecular weight and isoelectric point
(the pH at which a molecule carries no net electrical charge).