We used [sup.1]H-NMR-based metabolomics of aqueous
liver extracts to evaluate the metabolic profiles of WT male (Figure 3B) and female (Figure 3D) mice fed control or pesticide chow for 52 wk.
TABLE 1 PROTEIN CONCENTRATION AND SPECIFIC ENZYMATIC ACTIVITY OF THE
LIVER EXTRACTS Protein concentration (mg/mL) Liver sample Extract Control Lean Fatty Plain homogenate 31 [+ o -] 2 42 [+ o -] 2 68 [+ o -] 2 Metalloprotease 44 [+ o -] 1 41 [+ o -] 1 61 [+ o -] 1 Cathepsin 59 [+ o -] 2 60 [+ o -] 2 79 [+ o -] 1 Specific activity (a,b) (U/mg) Liver sample Extract Control Lean Fatty Plain homogenate 1.7 [+ o -] 0.1 1.4 [+ o -] 0.1 3.0 [+ o -] 0.1 Metalloprotease 12.4 [+ o -] 0.3 8.5 [+ o -] 0.3 20.5 [+ o -] 0.4 Cathepsin 10.2 [+ o -] 0.1 7.2 [+ o -] 0.3 21.5 [+ o -] 0.5 (a) Mean values from triplicates [+ o -] s.d.
We thank the cell and tissue imaging core facility of Institut Federatif de Recherche Therapeutique de Nantes for preparing tissue microarrays; Ghislaine Le Gall-Recule, Anses, Ploufragan, Pedro Esteves, and Joana Abrantes for providing whole
liver extracts of infected animals; and Lorenzo Capucci for providing monoclonal antibody 2G3.
Liver extract has also demonstrated remarkable results in the treatment of chronic hepatitis.
Liver extract with B-complex (Belamyl (a)) 10 ml i/m alternate day for 3 days as supportive therapy.
Typical chromatograms from calibrators, plasma extracts, and mouse
liver extracts are presented in Fig.
The massive hepatic necrosis was managed by administration of N-acetylcysteine and Silymarin along with ursodeoxycholic acid and
liver extract. Following treatment, focal hepatic lesion were found to decrease significantly and totally disappeared after 3 months based on ultrasonographic examination, though liver remained hyperechoic in echotexture due to hepatic lipidosis.
Gerson specified the "crude
liver Extract" (Lilly) which was quite dark brown.
ALT activity in the
liver extract was undetectable (<1 U/g of wet tissue, reference interval 50-70 U/g), whereas normal activities of alkaline phosphatase, [gamma]-glutamyltranspeptidase, and AST were found.