We used [sup.1]H-NMR-based metabolomics of aqueous liver extracts
to evaluate the metabolic profiles of WT male (Figure 3B) and female (Figure 3D) mice fed control or pesticide chow for 52 wk.
TABLE 1 PROTEIN CONCENTRATION AND SPECIFIC ENZYMATIC ACTIVITY OF THE LIVER EXTRACTS
Protein concentration (mg/mL) Liver sample Extract Control Lean Fatty Plain homogenate 31 [+ o -] 2 42 [+ o -] 2 68 [+ o -] 2 Metalloprotease 44 [+ o -] 1 41 [+ o -] 1 61 [+ o -] 1 Cathepsin 59 [+ o -] 2 60 [+ o -] 2 79 [+ o -] 1 Specific activity (a,b) (U/mg) Liver sample Extract Control Lean Fatty Plain homogenate 1.7 [+ o -] 0.1 1.4 [+ o -] 0.1 3.0 [+ o -] 0.1 Metalloprotease 12.4 [+ o -] 0.3 8.5 [+ o -] 0.3 20.5 [+ o -] 0.4 Cathepsin 10.2 [+ o -] 0.1 7.2 [+ o -] 0.3 21.5 [+ o -] 0.5 (a) Mean values from triplicates [+ o -] s.d.
We thank the cell and tissue imaging core facility of Institut Federatif de Recherche Therapeutique de Nantes for preparing tissue microarrays; Ghislaine Le Gall-Recule, Anses, Ploufragan, Pedro Esteves, and Joana Abrantes for providing whole liver extracts
of infected animals; and Lorenzo Capucci for providing monoclonal antibody 2G3.
has also demonstrated remarkable results in the treatment of chronic hepatitis.
with B-complex (Belamyl (a)) 10 ml i/m alternate day for 3 days as supportive therapy.
Typical chromatograms from calibrators, plasma extracts, and mouse liver extracts
are presented in Fig.
The massive hepatic necrosis was managed by administration of N-acetylcysteine and Silymarin along with ursodeoxycholic acid and liver extract
. Following treatment, focal hepatic lesion were found to decrease significantly and totally disappeared after 3 months based on ultrasonographic examination, though liver remained hyperechoic in echotexture due to hepatic lipidosis.
Gerson specified the "crude liver Extract
" (Lilly) which was quite dark brown.
ALT activity in the liver extract
was undetectable (<1 U/g of wet tissue, reference interval 50-70 U/g), whereas normal activities of alkaline phosphatase, [gamma]-glutamyltranspeptidase, and AST were found.