there was no visual evidence for allele dropout), 4) alleles were scored with minimum stutter, and 5) preference was given to loci that produced PCR amplicons in both species.
The likelihood ratio test of Excoffier and Slatkin (1998) was used to test for linkage disequilibrium between all pairs of microsatellite loci in Gulf Menhaden.
Primer sequences for all loci were as described previously (3,9,20,21).
We evaluated factors that potentially affect the reproducibility of hypervariable VNTR loci by using various PCR and capillary and manual electrophoresis separation conditions as described in the Materials and Methods.
After PCR amplification, the LOCI signal was typically read from reaction volumes of 40-100 [micro]L contained in 0.
Specific LOCI detection protocols are described with each of the assays below.
Future versions of LOCI will improve how government costs are allocated to residential, commercial and industrial sectors based on the demand each sector exerts on government services.
During fiscal year 1999, 50 LOCI projects were completed in 44 Georgia counties.
Multiple PCR products were analyzed in capillaries as follows: capillary 1 contained PCR products for loci 2, 4, 10, 16, 23, and 24; capillary 2 for loci 27, 31, 39, ETR-A, and ETR-B; capillary 3 for loci 20, 26, and ETR-C; capillary 4 for loci 0424, 0531, and 1955; capillary 5 for loci 2974, 3232, 3239, and 3690; and capillary 6 for loci 1982, 2163a, and 3336.
Molecular weights of PCR-generated fragments for loci 1982 and 3232 for some isolates exceeded 1 kb, and results of automated fragment analysis were inconsistent.
The technical ability to rapidly genotype such loci
in apes that develop human-like neuritic plaques, would help characterize the role of these genes in AD; the genotypic knowledge so gained could be used to develop more useful prospective studies of AD epidemiology in humans.
Launched in August 1995 as the first and largest site by college students for college students, LOCI
gives students an active, involving online "campus" to meet, be entertained, and exchange ideas.