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5 per cent had specific leptospiral antibodies, with titres ranging from 1:160 to 1:6400 by microscopic agglutination test (MAT) against different serovars, and 71 per cent seropositive cases were diagnosed by MAT with clinical suspicion of leptospirosis in 1996 (4).
Microscopic agglutination test (MAT) was performed as per protocol described in OIE (2008).
Assessment of the efficacy of an IgM-ELISA and Microscopic Agglutination Test (MAT) in the diagnosis of acute leptospirosis.
Leptospirosis case are diagnosed by serology, method is the microscopic agglutination test (MAT), in which live antigens representing different serogroups of leptospires are reacted with serum samples and then examined by dark field microscopy for agglutination[7].
All sera samples were tested for the presence of anti leptospiral antibodies using microscopic agglutination test (MAT) following standard methods (4).
Accurate diagnosis of leptospirosis has been hampered by the difficulty of the serologic testing with the gold standard microscopic agglutination test.
Blood samples were collected on third day and serum was subjected to microscopic agglutination test, which gave a positive result with a high titre of 1:800 for serovar autumnalis.
12) The Microscopic agglutination test (MAT) is the reference test for serological diagnosis of leptospirosis, because of its high sensitivity and specificity.