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14]C-cytidine incorporation into nascent DNA of cells confirmed that all compounds are pronounced inhibitors of DNA synthesis in the lower micromolar range.
In an in vitro reaction however, there's no pyrophosphatase around unless it's been added to the reaction, and so with each dNTP added to a nascent DNA, there's an additional PPi released into solution.
dNTPs, as the building blocks of nascent DNA strands by the DNA polymerase;