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The tissue is stained with human anti- mitochondria antibody (green), red is from the fluorescent Bangs particles and blue is Hoescht nuclear stain.
Using a fluorescent microsphere that excites and emits at one set of wavelengths, and a fluorescent nuclear stain that excites and emits at a different set of wavelengths, it was possible to image the microspheres in one channel and the fixed phagocyte cells in another channel.