Abbreviations: ACTH, adrenocorticotroph adenoma; GH, growth hormone adenoma; GH+, Pit-1 family member with strong growth hormone and a lesser degree of prolactin or thyroid-stimulating hormone (TSH) staining; Gon, gonadotroph adenoma; Null, null cell
adenoma; Pit1, Pit-1 family member (not otherwise specified); PlurGH, plurihormonal growth hormone adenoma; PlurGH+, plurihormonal growth hormone adenoma with lesser degrees of prolactin or TSH staining; PRL, prolactinoma; PRL+, Pit-1 family member with strong prolactin staining and lesser degree of growth hormone or thyrotropin staining; TSH+, TSH-producing adenoma with lesser degree of GH staining; Unk, unknown.
Lane M: [lambda] DNA digested with Pst I used as marker; Lane 1: Total RNA extracted from null cells
used as control RNA for reverse transcription and PCR amplification, no band corresponding to epo gene seen; Lanes 2, 3, 4 and 5: template RNA 0.5 [micro]g, 1.0 [micro]g, 2.0 [micro]g or 5.0 [micro]g respectively, the amplified band seen.
c, Occasional gonadotroph/ null cell
adenomas will demonstrate clustering of cells, paralleling the perivascular pseudorosette-like formation that can occur on permanent sections.