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At the egg cell membrane, there were microvilli projecting outward into the perivitelline space (Fig.
Some of the oocytes were striped off expanded cumulus cells for visualization of the extruded first polar body in the perivitelline space.
It was reported (1) that Heliocidaris eggs develop beneath the tightly fitting perivitelline space and form a wrinkled blastula in order to increase the ratio of the embryonic surface to the available volume.
Sperm injected into the perivitelline space with these caps still in place cannot fertilize the egg, she says.
4 mm with an ocular micrometer and having one or more of the morphological characteristics corresponding to cobia eggs--single, large (300 to 600 [micro]m), pigmented oil droplet, heavily pigmented embryo, or narrow perivitelline space (Ditty and Shaw, 1992; Ditty, 2006)--were removed and preserved in 10% neutral buffered formalin.
There was an extrusion of the first polar body from the egg plasma membrane into the perivitelline space at 8 9 min (Fig.
Within a minute postinsemination, when the fertilization envelope could be identified, a number of particles were observed in the perivitelline space (perivitelline-space particles) (Fig.
After enucleation, the nuclear donor cells were deposited into the perivitelline space of enucleated oocytes to facilitate close membrane contact for subsequent fusion.
However, once the fertilization membrane is raised, the perivitelline space exceeds the length of the nuclear filament, preventing other sperm from penetrating the egg.