Clinical trials revealed that about 20% aneuploidies, detected by polar body
biopsy and confirmed by blastomere biopsy, turned out nonexistent at blastocyst stage due to self-correction .
Percentage of bovine oocytes with cumulus expansion and first polar body
extrusion, matured in vitro either at 36.5[degrees]C or 38.5[degrees]C maturation temperatures Culture Oocyte Cumulus expansion First polar body
temperature quality ([degrees]C) n % n % 36.5 Grade A 623 96.2 78 79.5 (a) Grade B 687 89.8 108 63.0 (b) 38.5 Grade A 622 95.8 82 85.4 (a) Grade B 751 90.2 112 74.1 (a) (a,b) Different letters in the same column indicate significant difference (p < 0.05).
Figure 2K-0 shows the zygotes just after polar body
formation viewed from the lateral side.
Cytochalasin D inhibition of polar body
formation, followed by washout
After extrusion of the first polar body
(PB1), the second meiotic spindle appeared.
Egr3 siRNA (25 [micro]M) was microinjected into GV oocytes and rates of GVBD and polar body
extrusion were monitored.
The polar body
extrusion rate was not significantly different among the control and the treatment groups at 1 or 10 [micro]g/mL, but it was significantly lower (p < 0.05) in the group containing 50 [micro]g/mL TF (59.2% [+ or -] 7.9%) compared with the control (80.5% [+ or -] 3.5%) and the other treatment groups (Table 1).
Optimization of tetraploid induction in the Pacific oysters, Crassostrea gigas, using the first polar body
as a natural indicator.
formation is of interest both as a fundamental process in sexual reproduction and as an extreme example of unequal cytokinesis in cell biology.
An indication of successful fertilization was first demonstrated by the presence of the first polar body
, which appeared at 8 9 min after incubation (Fig.
Extensive studies of meiotic spindle motility in mouse oocytes have revealed a detailed series of movements during meiotic asymmetric divisions: during first meiotic division, the MI spindle forms initially at the center of the oocyte and migrates toward the periphery; the first polar body
(PB1) is extruded after microtubule-cortex attachment (Maro et al., 1984; Longo and Chen, 1985; Maro et al., 1986; Verlhac et al., 2000; Maro and Verlhac, 2002; Wang et al., 2008).
Inhibition of polar body
formation required at least 1 [micro]M CD, but percent inhibition of polar bodies was difficult to judge, since they could only be seen if present along the edge of the egg.