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Briefly, 6 [micro]m sections of paraformaldehyde-fixed liver tissue were postfixed with precooled fixative (ethanol/acetic acid) for 5 min at -20[degrees]C.
The postfixed animals were rinsed with 8% sucrose aqueous solution and en bloc stained with 1% aqueous uranyl acetate for 2 h at room temperature.
* The majority of these were postfixed brachial plexus (62%) (Fig.
For analysis by transmission electron microscopy (TEM), cells were prefixed with glutaraldehyde, as above, and then postfixed with the Os[O.sub.4] solution overnight, at 4[degrees]C.
After light-activated curcumin (2.5 [micro]M, 3 J/[cm.sup.2]), bacterial cells were fixed for 1 day in 2% glutaraldehyde and postfixed with 2% Os[O.sub.4], dehydrated with graded alcohol and embedded with Epon 812 (Electron Microscopy Sciences, Fort Washington, PA, USA).
Specimens are postfixed in 1% osmium tetroxide, en bloc stained with 4% uranyl acetate, dehydrated through a graded series of alcohol and propylene oxide, and embedded in a mixture of Epon substitute and Araldite.
We removed the brains and postfixed them in 4 percent paraformaldehyde for 24 h, followed by incubation in cryoprotectant.
The material was then washed in sodium phosphate buffer, and postfixed using 1% Osmium tetroxide (Os[O.sub.4]).
Then, their brains were separated by usual dissection methods and postfixed in 4% paraformaldehyde for 2h.
The cells were washed in wash buffer containing 4% sucrose in PBS and postfixed in 1% osmium tetroxide in PBS for 1 h at 4[degrees]C.
For routine paraffin sectioning, brain and spinal cord tissues were postfixed in 4% PFA for overnight.
A portion of the repeat FNA sample of the neck mass was sent for electron microscopy which was fixed in phosphate-buffered glutaraldehyde, postfixed in osmium tetroxide, embedded in Epon and sections were cut and stained with uranyl acetate / lead citrate.