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Points of Concern for MRM-Based Protein Quantification
MRM-based quantification relies heavily on the monitoring of selected "proteotypic" peptides, or peptides that are precise and highly reproducible representations of the intact protein of interest, and their product ions (Fig.
Quantification of intermediate-abundance proteins in serum by multiple reaction monitoring mass spectrometry in single-quandrupole ion trap.
Isotope dilution mass spectrometric quantification of specific proteins: model application with apolipoprotein A-I.
Absolute quantification of the model biomarker prostate-specific antigen in serum by LC-MS/MS using protein cleavage and isotope dilution mass spectrometry.

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