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In each brain studied, the volume of nucleus rotandus and red nucleus was determined on the right and left sides.
ROIs were drawn manually by the author and coauthor of this work in SPIN based on their anatomical locations of deep grey matter of brain; caudate nucleus (CN), red nucleus (RN), globus pallidus (GP), putamen (PUT), thalamus (THA), substantia nigra (SN) and white matter (WM).
The typical locations of lesions by MR imaging in patients with MIE are the cerebellar dentate nuclei, midbrain (tectum, red nucleus, tegmentum around periaqueductal gray matter), dorsal pons, dorsal medulla, and corpus callosum (splenium); and these were always bilateral and symmetric.