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Similar to Endo IV, the pro-Rp rather than the pro-Sp nonbridging oxygen of the scissile phosphate is intolerant of thio-replacement.
Two conserved His residues surrounding the scissile phosphate haven shown to be important for the general acid-and-base catalysis, and the His mutations can be rescued by imidazole.
In the proposed mechanism for sedolisin, Glu80 acts as a general base to accept a proton from Ser287 during the nucleophilic attack and then as a general acid to protonate the leaving group during the cleavage of the scissile bond.
N-terminal analysis has revealed the margins of the capsid proteins and the corresponding scissile bonds within the structural polyprotein (Valles and Hashimoto 2008).
So, they've been revving up the thrill-ometer (and added 3D, natch) for this ultimate road movie in which Lightning McQueen (voiced by Owen Wilson) and his best friend Mater compete in the World Grand Prix in Tokyo - and get mysteriously recruited by British intelligence vehicle Finn Scissile (Michael Caine) in the process.
The processing of Gag is regulated by the residues flanking the scissile bonds.
The playfulness of the cento, which Ausonius describes as a game, represents the master text as a scissile surface and in so doing suggests that the authority vested in the master text is only skin deep.
Substrate specificity of the metalloproteinase pregnancy-associated plasma protein-A (PAPP-A) assessed by mutagenesis and analysis of synthetic peptides: substrate residues distant from the scissile bond are critical for proteolysis.
Peptidases in which the nucleophile that attacks the scissile peptide bond is the sullhydryl group of a cysteine residue are known as cysteine-type peptidase.