Above table shows that majority of the academic staff who were not using the serial section
did so because of lack of recent journal in their area of interest.
We present 2 cases with lepidic growth, mimicking papillary pattern, and use a 3-dimensional (3D) reconstruction and serial sections
to arrive at the correct diagnosis.
A minimum of 50 well-oriented, not crushed, H&E-stained serial sections
with sufficient submucosa are required to exclude the presence of GCs and suggest the diagnosis of HSCR (Figure 4).
Periglomerular fibrosis is not always associated with complete occlusion of the glomerulotubular junction, as seen on serial sections
. Sometimes only narrowing of the junction was seen, associated with prominent atrophy of the adjoining tubule.
Twenty to 40 5-[micro]m-thick serial sections
were cut from each block and immunostained with antikeratin CAM5.2 (Becton Dickinson, San Jose, Calif) on an automated immunostainer (Ventana Medical Systems, Tucson, Ariz) using standard avidin-biotin complex methods with diaminobenzidine as the chromogen.
from the same patient were treated with ammonia-ethanol (E through H) or AutoFluo Quencher for 10 minutes (I through L), 30 minutes (M through P), 60 minutes (Q through T), 90 minutes (U through X).
Three-dimensional (3D) reconstructions of serial sections
of three embryos from CS14 to CS16 were performed using AMIRA software version 5.2 (AMIRA International Limited, Australia) to display the morphological differences in the embryonic hearts.
The system incorporates a 300-slide autoloader that supports multiple 1x3 and 2x3 inch slides and registration of serial sections
for 3D reconstruction, along with 6x8 inch slides for large specimen imaging.
(small wedge, defect open; small wedge, defect closed; large wedge, defect open; large wedge, defect closed [equivalent to a protruding ear]; and pinnectomy) were undertaken, and the sound intensity changes assessed....
Three sets of 15 consecutive serial sections
, each measuring 3-5 [micro]m in thickness and mounted on separate slides, were prepared from each case at one sitting, taking care to always use the same microtome in order not to lose any tissue in between sections.
Transverse serial sections
of whole juvenile pearl oysters were prepared.
of cervical and thoracic segments of 6wks.