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The sickling test was positive in all the sickle cell disorders but the only difference between sickle cell disease and sickle cell trait was that this test showed sickling of the red blood cells a little bit late in carrier states.
Total bilirubin was 19 umol/ and alanine trans-aminase (ALT) was 155 u/l, Sickling test was positive.
Haemoglobin was estimated by Sahli's Haemoglobinimeter method (7,8) and sickling test was also performed.