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In the present study, we developed an ex vivo test system based on precision-cut adrenal slice culture to examine metabolism-dependent binding, ultrastructural changes, and effects on steroid synthesis in mouse adrenal tissue exposed to Me[SO.
To visualize dendritic arbors, slice cultures were biolistically transfected with plasmid-encoding tomato fluorescent protein (TFP), which fills the entire cell, using a Helios Gene Gun (BioRad, Hercules, CA), according to the manufacturer's protocol.
Presentation P-187, entitled Tramiprosate Is Neuroprotective and Reduces the Levels of Secreted Amyloid-ss in Organotypic Hippocampal Slice Cultures, will be presented by lead author Mounia Azzi.