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Mycoparasitism interaction of each antagonist against fungal pathogen was determined using slide culture techniques. Agar blocks of the antagonists and fungal pathogens were grown equidistantly with a distance of 1 cm in a glass slide containing a very thin water agar.
In the present study, paraffin slide culture technique was also used for recovery of Nocardia species from sputum samples, however we did not encounter even a single case of nocardiosis.
Preparation of in house paraffin slide culture technique