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Use of urea (5M) induced tertiary conformations of these proteins improved sero-detection on immune blots of cysticercus antibodies among patients with solitary cyst infections to 71% from 62% using antigens not treated with urea.
In contrast, one of the following scenarios was expected for cases in three of five persons in the ES group who did not have SCN and were seronegative: 1) the case was not due to TsCysti, 2) the case was caused by TsCysti but without antibody response, rather common in cases of a solitary cyst, or 3) the case was caused by TsCysti with calcified cysts and without antibody response.
6) A solitary cyst in the pelvic cavity can be considered primary only when no other cysts are present.