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The iron content in the filtrate was determined by the method [11] based on the formation of complexes with sulfosalicylic acid.
Therefore, we believe that the confirmatory sulfosalicylic acid test should be performed in all cases of pathological microscopic findings in the urine (presence of [greater than or equal to]10 fresh erythrocytes/[micro]L and/or [greater than or equal to]1 dysmorphic erythrocyte/ [micro]L and/or [greater than or equal to]10 leukocytes/[micro]L and/or [greater than or equal to]1 cylinder (except hyaline) and/or [greater than or equal to]1 nonsquamos epithelial cells/[micro]L and/or [greater than or equal to]100 bacteria/[micro]L) even if the test strip examination is negative for proteinuria.
In few cases, the dipstick analysis showed false negative results in comparison to the results of the quantitative analysis done by the sulfosalicylic acid colorimetric method.
Use of sulfosalicylic acid in the detection of proteinuria and its application to hypertensive problems in pregnancy.
This study ("Comparison of urine dipstick, sulfosalicylic acid, urine protein-to-creatinine ratio, and species-specific ELISA methods for detection of albumin in urine samples of cats and dogs," Journal of American Veterinary Medical Association, 2010) evaluated the use of dipstick, sulfosalicylic acid (SSA), and urine protein-to-creatinine ratio (UP:C) methods for use in detection of albumin in urine.
Briefly, juice samples were acidified with sulfosalicylic acid to precipitate any intact protein before analysis.
Proteinuria was considered positive if present in urinalysis, sulfosalicylic acid test or time urine collection if higher than 4 mg/[m.
Sulfosalicylic acid is an aromatic sulfur-oxygen compound.
I mixed 10 [micro]L homogenate from the peroxidation assay with 100 [micro]L 5% sulfosalicylic acid (SSA) to denature proteases.
For confirmatory testing of other reagent strip constituents, commonly used tests include the sulfosalicylic acid test (SSA) for protein, and the Ictotest Tablet Test (Bayer) for bilirubin.
5 g fresh leaf sample was mixed in 10 mL of 3% sulfosalicylic acid and the material was filtered and two mL from the filtered sample was taken and reacted with two mL acid ninhydrin solution in a test tube.

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