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Histologic and in situ hybridization analyses of livers from cynomolgus macaques infected with Lassa virus (LASV) (1 x [10.sup.4] 50% tissue culture infective dose [[TCID.sub.50]]) and treated 1 time daily with favipiravir (treated) or vehicle only (control).
Key words: Immunogen, adjuvant, mean tissue culture infective dose, keeping quality, rabbits.
selectivity index; TCID5J), 50% tissue culture infective dose; TM8, 3'.3',5'.5'-tetrumethyIbenzidine.
With serially diluted positive control RNA, the detection limits of the conventional and quantitative RT-PCR assays were found to be 1.0 x [10.sup.-4] [TCID.sub.50] (median tissue culture infective dose) per reaction and 2.0 x [10.sup.-3] [TCID.sub.50] per reaction, respectively (Fig.
MDCK cells were infected with serially diluted pseudovirus stock, and the infectivity reflected by the relative luciferase activity (RLA) was determined as the median (50%) tissue culture infective dose, according to the method of Reed and Muench.