unretarded


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The 79 kDa haemolytically inactive pro-VCC, which eluted unretarded leaving LPS and lipid vesicles bound to the column, adsorbed quantitatively to a phenyl-Sepharose CL-4B column in buffer A and was subsequently desorbed by 60 per cent ethylene glycol.
It was shown that a fraction of the bentonite colloids moved always unretarded, compared to the water flow, other colloids eluted retarded and a colloid fraction was filtered in the medium.
Passage in reverse order over dodecyl agarose removes nearly all of the remaining protein, but the FRED elutes almost unretarded.