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Previously published methods for RHD zygosity testing have included real-time PCR assessment of RHD gene dosage, assessment of the hybrid Rhesus box found in D- individuals with the RHD gene deletion genotype and allele-specific PCR methods, as well as mass spectrometry-based methods (2, 4-10).
The authors are grateful to Professor Propping for determining zygosity. They also thank the following institutions for supporting them in recruiting the twins: Pfalzklinik Landeck Klingenmunster, Psychiatrisches Krankenhaus Christophsbad Goppingen, Psychiatrische Abteilung, Stadtisches Krankenhaus Karlsruhe, Abteilung fur Psychiatrie und Psychotherapie, SRH Klinikum Karlsbad-Langensteinbach, Psychiatrische Abteilung Universitat Bonn, Zentralinstitut fur Seelische Gesundheit Mannheim, and Zentrum fur Psychiatrie Nordbaden.
The sample included 41 pairs of twins (82 individuals) who were divided by their zygosity into 25 pairs of MZ twins (50 individuals: 28 females and 22 males) and 16 pairs of DZ twins (32 individuals: 18 females and 14 males), aged 8 to 26 yrs old; all of these twin pairs resided in the same households.
Furthermore, due to the overrepresentation of families with DZ twins, zygosity comparisons could not be completed.
Amyloid-related imaging abnormalities with edema occurred in up to 27% of APOE-epsilon-4 carriers, depending on their zygosity It also appeared in up to 14% of noncarriers; the relationship was dose dependent.
Amyloid-related imaging abnormalities with edema (an adverse event associated with immune response directed toward plaques) occurred in up to 27% of APOE-ep-silon-4 carriers, depending on their zygosity. It also appeared in up to 14% of noncarriers, the relationship was dose dependent.
Separate analyses by zygosity (crude OR = 0.5, 95% CI: 0.05-5.5 for MZ pairs; crude OR = 0.44, 95% CI: 0.14-1.44 for DZ pairs) found associations in the protective direction, although nonsignificant due to a limited number of pairs.
However, while the twins had said that they were identical, and understood from their mother that they were identical, according to Twin A no DNA testing had ever been performed to confirm zygosity. Research shows that the closest correlation to DNA test results on monozygosity is the twins' own opinion, which was reported to be 95% correct.
grandparentage, siblingship, twin zygosity, and other family-related
Atopic dermatitis was diagnosed by the question "has your child ever had eczema in the folds of the elbows or knees?" Zygosity was determined by information regarding the twins' similarity to family, friends, teachers, and relations.
Sensory method facilitate the identification of aromatic and nonaromatic genotypes while molecular markers assist to identify specific allele but single tube allele specific amplification guides to identify zygosity (Homozygous or heterozygous for fgr gene) of individuals.